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产品名称:(衍生化 串联质谱法LC-MS/MS)

产品型号:55000-XS

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英文名:MassChrom®AminoAcidsandAcylcarnitinesfromdriedblood-LC-MS/MS供应商:祥生兴业规格:960analyses新生儿遗传代谢疾病诊断串联质谱分析方法检测试剂盒

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55000-XS(衍生化 串联质谱法LC-MS/MS)的详细资料:

温馨提示:仅用于科研,不可用于临床治疗

  • 英文名:

    MassChrom® Amino Acids and Acylcarnitines from dried blood - LC-MS/MS

  • 供应商:

    祥生兴业

  • 规格:

    960 analyses

    新生儿遗传代谢疾病诊断串联质谱分析方法检测试剂盒。

    • Dried blood spot controls included

    • Multiple diagnoses in a single run

    • High selectivity and sensitivity by MS/MS detection

    • New! Succinylacetone Upgrade Set

    The Chromsystems reagent kit for the determination of amino acids and acylcarnitines from dried blood spot samples using tandem mass spectrometry is intended for the early diagnosis of amino acid and fatty acid metabolic disorders during newborn screening.

    In Germany, newborn screening is regulated by law. Twelve diseases are defined, eight of them to be determined by tandem mass spectrometry. As therapies for only these specific diseases are known, the diagnosis of other diseases is prohibited. Included in these diseases are defects of amino acid metabolism (phenylketonuria and maple syrup urine disease), defects of fatty acid metabolism (Medium and Very Long Chain Acyl-CoA Dehydrogenase Deficiencies) and organoacidurias (isovaleric acidemia). If not detected, these metabolic errors may impair the newborn severely and irreversibly within the first days of its life.

    Specifications (analysis without SUAC):

    Linearity: Amino acids: 2000-2400 µmol/l
    (Acyl-)carnitines: 25-200 µmol/l
    Limit of quantification: Amino acids: 2-16 µmol/l
    (Acyl-)carnitines: 0.1-1.6 µmol/l
    Recovery: Amino acids: 67-90 %
    (Acyl-)carnitines: 81-97 %
    Intra-assay: Amino acids: 3-9 %
    (Acyl-)carnitines: 4-16 %
    Inter-assay: Amino acids: 6-11 %
    (Acyl-)carnitines: 7-20 %
    Analysis time: < 2 min

    Specifications (analysis with SUAC):

    Linearity: Amino acids with SUAC: 250-2500 µmol/l
    (Acyl-)carnitines: 11-240 µmol/l
    Limit of quantification: Amino acids with SUAC: 0.5-5 µmol/l
    (Acyl-)carnitines: 0.05-0.5 µmol/l
    Recovery: Amino acids with SUAC: 51-94 %
    (Acyl-)carnitines: 62-89 %
    Intraassay: Amino acids with SUAC: 4-10 %
    (Acyl-)carnitines: 4-20 %
    Interassay: Amino acids with SUAC: 5-9 %
    (Acyl-)carnitines: 8-28 %
    Analysis time: < 2 min

    Cutoff thresholds

    Amino acids: 22-743 µmol/l
    Free carnitine: 50-60 µmol/l
    Acylcarnitines: 0.1-48 µmol/l
    SUAC: 1.16 µmol/l

    LC-MS/MS parameters

    LC-MS/MS (tandem MS). No HPLC column is required.

    Injection volume: 10 µl
    Flow gradient: 20-600 µl/min
    Autosampler rinsing solution: Rinsing Solution (55007)


    Mass transitions of analytes and internal standards

    Amino acids Neutral Loss Scan 102
    Alanine 146 > 44
    Alanine-D4 150 > 48
    Aspartic acid 246 > 144
    Aspartic acid-D3 249 > 147
    Glutamic acid 260 > 158
    Glutamic acid-D5 265 > 163
    Leucine 188 > 86
    Leucine-D3 191 > 89
    Methionine 206 > 104
    Methionine-D3 209 > 107
    Phenylalanine 222 > 120
    Phenylalanine-D5 227 > 125
    Tyrosine 238 > 136
    Tyrosine-D4 242 > 140
    Valine 174 > 72
    Valine-D8 182 > 80
    MRM
    Arginine 231>70
    Arginine-D7 238 > 77
    Citrulline 232 > 113
    Citrulline-D2 234 > 115
    Glycine 132 > 76
    Glycine-13C2-15N 135 > 79
    Ornithine 189 > 70
    Ornithine-D6 195 > 76
    Proline 172 > 116
    Proline-D7 179 > 123
    SUAC 211 > 109
    SUAC-13C5 216 > 114
    (Acyl-)carnitines Parent Ion Scan 85
    Carnitine 218 > 85
    Carnitine-D9 227 > 85
    C2-Carnitine 260 > 85
    C2-Carnitine-D3 263 > 85
    C3-Carnitine 274 > 85
    C3-Carnitine-D3 277 > 85
    C4-Carnitine 288 > 85
    C4-Carnitine-D3 291 > 85
    C5-Carnitine 302 > 85
    C5-Carnitine-D9 311 > 85
    C5DC-Carnitine 388 > 85
    C5DC-Carnitine-D6 394 > 85
    C6-Carnitine 316 > 85
    C6-Carnitine-D3 319 > 85
    C8-Carnitine 344 > 85
    C8-Carnitine-D3 347 > 85
    C10-Carnitine 372 > 85
    C10-Carnitine-D3 375 > 85
    C12-Carnitine 400 > 85
    C12-Carnitine-D3 403 > 85
    C14-Carnitine 428 > 85
    C14-Carnitine-D3 431 > 85
    C16-Carnitine 456 > 85
    C16-Carnitine-D3 459 > 85
    C18-Carnitine 484 > 85
    C18-Carnitine-D3 487 > 85

    Sample preparation (general)

    • Punch a 3 mm dried blood spot disk out of the filter card into a well of a microtiter plate (or a filter plate).
    • Add 200 µl of the reconstituted Internal Standard, seal with a protective sheet and agitate for 20 min at 600 rpm and room temperature.
    • Transfer the supernatant into a new microtiter plate. When using a filter plate draw through the supernatant into the well plate by centrifugation (4000 rpm, 2 minutes). Discard the filter plate.
    • Evaporate at 60 °C and 600 rpm to dryness.
    • Add 60 µl Derivatisation Reagent, seal microtiter plate with a protective sheet and incubate 15 min at 60 °C and 600 rpm.
    • Evaporate at 60 °C and 600 rpm to dryness.
    • Add 100 µl Reconstitution Buffer and agitate 1 min at 600 rpm.
    • Inject 10 µl into the LC-MS/MS system.

    Sample preparation (succinylacetone)

    • Punch a 3 mm dried blood spot disk out of the filter card into a 96 well plate (or a filter plate).
    • Add 200 µl Internal Standard, seal with a protective sheet and agitate for 20 min at 600 rpm and room temperature.
    • Transfer the supernatant into a new 96 well plate. When using a filter plate draw through the supernatant into the 96 well plate by centrifugation (4000 rpm, 2 minutes).
    • Add 150 µl Internal Standard Succinylacetone to the remaining blood spot and agitate for 30 min at 60 °C.
    • Pool the supernatants and evaporate at 60 °C and 600 rpm to dryness.
    • Add 60 µl Derivatisation Reagent, seal microtiter plate with a protective sheet and incubate 15 min at 60 °C and 600 rpm.
    • Evaporate at 60 °C and 600 rpm to dryness.
    • Add 200 µl Reconstitution Buffer and agitate 1 min at 600 rpm.
    • Inject 10 µl into the LC-MS/MS system.

    Order no. Product
    55000 MassChrom® Reagent kit for the determination of Amino Acids and Acylcarnitines from dried blood spot samples (LC-MS/MS) with regular 96 well plates.
    For 960 analyses.
    55000/F MassChrom® Reagent kit for the determination of Amino Acids and Acylcarnitines from dried blood spot samples (LC-MS/MS) with 96 well filter plates.
    For 960 analyses.
    Separately available components
    55001 Mobile Phase, 1000 ml
    55002 Mobile Phase, 10 x 1000 ml
    55004 Internal Standard (lyoph.), 4 x 50 ml
    55044 Internal Standard for Succinylacetone, 3 x 50 ml
    55004/T Internal Standard (lyoph.), 50 ml
    55044/T Internal Standard for Succinylacetone, 50 ml
    55005 Derivatisation Reagent, 30 ml
    55006 Reconstitution Buffer, 100 ml
    55007 Rinsing Solution, 1000 ml
    55008 Extraction Buffer, 200 ml
    55010 96-Well-Plates, 10 pcs.
    55011 Protective sheets for 96 well plates, 10 pcs.
    55057 96-Well-Filter-Plates, 5 pcs.
    Succinylacetone Upgrade Set
    55111 Succinylacetone Upgrade Set for 960 analyses, consisting of:
    Internal Standard for Succinylacetone, 3 x 50 ml
    Reconstitution Buffer, 100 ml
    Startup accessories
    55013 Pierceable adhesive seal for 96 well plates, 10 pcs.
    55015 Restrictor Capillary, 1 pc.
    55016 Adapter collar for centrifugation, 2 pcs.
    55098 Tuning Mix for Succinylacetone, 1 ml
    55099 Tuning Mix incl. all analytes, 2 ml
    55033 PEEK prefilter, 5 µm, 5 pcs.
    15010 PEEK prefilter housing, 1 pc.
    Chromsystems quality control material (filter spot):
    0191 MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Bi-Level (I+II), 2 x 3 Spots
    0192 MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level I, 1 x 3 spots
    0193 MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level II, 1 x 3 spots




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